The DNA Assembly Cloning Kit is a simple, fast, and efficient seamless cloning kit for DNA that can direct the insertion fragment to any site on any vector. Linearize the vector in any manner, and introduce the end sequence of the linearized vector into the 5' ends of the insertion fragment's forward/reverse PCR primers, so that the 5' and 3' ends of the PCR product carry sequences consistent with both ends of the linearized vector (25-40 bp).This type of PCR product, which has a carrier end sequence at both ends, can be mixed with linearized vectors in a certain ratio. Under the catalysis of recombinase, transformation can be carried out by reacting at 50°C for 15 minutes to complete directional cloning.