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Alpaca VHH Antibody Discovery
At Smart Lifesciences, has developed proprietary procedures for nanobody discovery. We optimized the immunization schedule and screening proceddes to target-focused immune response and ensure the successful isolation of high-affinity alpaca nanobody within a short turnaround time.
Advantages of nanobody include, stability at hight temperature & pH, VHHs could recognize antigenic sites that are normally not recognized by IgG antibodies, the small moleculara size could help with rapid tissue penetration and conjugation applications, low cost for large-scale production. All positice VHH generated under your project will belong to you.
· Highlights
High throughput and shot timeline - single cell within 1 week
Animal immunization (carrier protein to increase the anti-serum titer more than 20%, self-developed adjuvant)
Project success rate - 100%.
One stop solution - from gene to antibody
Extensive experience - 13+ years
· VHH antibody discovery process
Antigen preparation
Immunization
B cell isolation
Screening
lgG sequencing
rAb expression
· Service SpecificationsPhases TAT(weeks) Descriptions Phase 0: Antigen preparation Peptide/Protein preparation 3-4 / Phase I: Animal immunization Three immunizations with conventional method & report only 8-10 ELISA report after the 3rd immunization Serum collection and PBMC isolation 8-10 ELISA report after the 3rd immunization Phase II: Positive clones screening Option 1: Phage display and bio-panning 2-8 ELISA report of positive clones Option 2: B cell cloning & ELISA serttning 2-8 ELISA report of positive clones Phase III:Sequencing, recombinant VHH expression and validation Antibody sequencing and construction 2-4 Antibody sequencing and alignment reports Small scale recombinant VHH production and validation 2-4 0.1mg recombinant VHH/clone (SDS-PAGE>90%, endotoxin level<5EU/mg) Note:
1.Starting material: antigen protein>5mg, purity>85%, conc.>1mg/ml.
2.Starting material: antigen peptide>15mg, purity>90%.· Project Case Study - anti-HA VHH
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Rabbit Monoclonal Antibody Discovery
Smart's customized rabbit monoclonal antibody development service could offer high affinity, quality, specific, and sensitive monoclonal antibodies against a wide range of antigens. By using proprietary B cell cloning technology, this service provides several advantages over traditional mouse monoclonal antibody development.
Smart’s B-cell cloning technology has a high success rate against difficult targets such as PTM site, small molecule,antibody drugs, etc. Our advanced, proprietary rabbit monoclonal antibody discovery technology involves the isolation and cell culture of rabbit B cells to generate high-specificity, high-affinity antibodies to meet customers’ downstream applications.
In a word, this platform could offer higher specificity, affinity and high success rate, the ability to recognize more epitopes, and the capability to large scale antibody production and wide applications in scientific research, diagnostics, and therapeutics.
· HighlightsAnimal immunization (carrier protein to increase the anti-serum titer more than 20%, self-developed adjuvant)
Low price - almost same with mouse mAb
Project success rate - 100%
One stop solution - from gene to antibody
Extensive experience - 13+ years
· Rabbit monoclonal antibody discovery processAntigen preparation
Immunization
B cell isolation
Screening
lgG sequencing
rAb expression
· Service SpecificationsPhases TAT(weeks) Descriptions Phase 0: Antigen preparation Peptide/Protein preparation 3-4 / Phase I: Animal immunization Three immunizations with conventional method (three rabbits) & report only 6~8 ELISA report after the 3rd immunization Phase II: B cell cloning and screening One round of B cell cloning&report only 2 Positive clone report for up to 20 clones Phase III:Sequencing, validation and recombinant antibody production Antibody sequencing and validation 2 Antibody sequencing and alignment reports for up to 5 clones Small scale recombinant antibody production for five clones 3~4 0.1mg recombinant antibody/clone from up to 5 clones Optional: Antibody sequencing and small scale antibody production for one extra clone / Antibody sequencing and alignment report and 0.1mg recombinant antibody/clone Phase IV: Recombinant Antibody production Option1: Recombinant Antibody production 3 0.1-0.5mg/clone Option2: Recombinant Antibody production 4~6 1-2mg/clone Note:
1.Starting material: antigen protein>5mg, purity>85%, conc.>1mg/ml.
2.Starting material: antigen peptide>15mg, purity>90%.· Project Case Study - anti-HA VHH
· Project case study - anti target C ELISA kit
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Mouse Monoclonal Antibody Discovery
The mouse monoclonal antibody is a type of antibody produced by a single clone after immunization derived from mice. Monoclonal antibodies have a much higher specificity and lower between-batch variability compared to pAbs, which is widely used in such as research and development (R&D), diagnostics, and therapeutics.
Smart's custom mouse monoclonal antibody development service provides a comprehensive solution for generating high-quality monoclonal antibodies against any target of interest. The service includes immunogen design and synthesis, immunization, hybridoma generation, screening, and antibody production.With Smart's fully customizable monoclonal antibody generation services, you have complete control over every stage of the process, allowing you to modify your approach, restart a phase, or terminate the project if necessary. Avoid the risks associated with a rigid protocol and choose our fully customizable service that provides the ultimate flexibility. Partnering with Smart's years of expertise in custom antibody development can help you achieveproject success.
· HighlightsAnimal immunization (carrier protein to increase the anti-serum titer more than 20%, self-developed adjuvant)
Project success rate - 100%
One stop solution - from gene to antibody
Extensive experience - 13+ years
· Mouse monoclonal antibody discovery process
Antigen preparation
Immunization
Screening
B cell isolation
lgG sequencing
rAb expression
· Service SpecificationsPhases TAT (weeks) Descriptions Phase 0: Antigen preparation Peptide/Protein preparation 3-4 / Phase I: Animal immunization Three immunizations with conventional method (five mice) 6-8 ELISA report after the 3rd immunization Phase II: Cell fusion and sub cloning screening One cell fusion with up to 15 plates 5 Positive clone report for up to 10 clones
2 vials of each sub-cloned hybridoma cell line from 3-5 positive primary cell linesSubcloning for 3-5 clones Phase III: Antibody production Option 1: Antibody production in low IgG culture medium and protein A/G purification 3 0.1-0.5mg/clone Option 2: Ascites production and protein A/G purification 2-3 1-2mg/clone Note:
1.Starting material: antigen protein>3mg, purity>85%, conc.>1mg/ml.
2.Starting material: antigen peptide>10mg, purity>90%.· Project case study - anti MBP mAb
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Polyclonal Antibody Discovery
Smart Lifesciences comprehensive polyclonal antibody services include production of custom polyclonal antibodies in mouse, rabbit and other species. Smart provides one-stop solutions for your custom polyclonal antibody production, taking your project all the way from antigen design, peptide synthesis, and animal immunization, to antibody purification and QC with ELISA test.
· HighlightsOne stop solution
R&D carrier protein
High purification yield
High success rate
· Polyclonal antibody discovery processAntigen preparation
Immunization
B cell isolation
Screening
· Service SpecificationsPhases TAT(weeks) Descriptions Phase 0: Antigen preparation Peptide/Protein preparation 3-4 / Phase I: Animal immunization Three immunizations with conventional method (two rabbits) & report only 6~8 ELISA report after the 3rd immunization Phase II: Polyclonal antibody purification Purification for all rabbits by antigen affinity column 2 Purified antibody from both rabbits and ELISA report Note:
1.Starting material: antigen protein>5mg, purity>85%, conc.>1mg/ml.
2.Starting material: antigen peptide>15mg, purity>90%.· Project case study - Target A rabbit pAb
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Antibody Sequencing Service
Smart Lifesciences offers a fast and professional variable and full length sequencing service for customer’s valuable monoclonal antibodies. We could sequence from multi species antibody producing cell line, which could be railored to requirements for various applications, including monoclonal antibody (mAb) engineering, functional optimization and drug development. So, in order to prevent the loss of genetic information, sequencing is a powerful tool for identifying antibodies produced by hybridoma cells.
· HighlightsProject success rate - 100%
One stop solution - from cell to recombinant antibody
Multi species of B cells - mouse, rabbit, alpaca, human.
· Hybridoma IgG sequencing process
RNA extraction
CDNA amplification
Vector cloning
Validation
Report & delivery
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HTP Antibody Production Service
As the purificatioon expert, Smart Lifesciences apply rapid purification platforms for the process from target gene to candidates by using HEK293 or CHO cells, both known for their efficiency and fast in recombinant protein expression. High throughput platform (HTP) offers the most cost-effective option for small-scale recombinant antibody production. For Smart’s high throughput transient expression technology, which starting from customer-supplied sequences/plasminds to purified protein/antibody products, we could be completed in as short as 7 business days!
All the process could be finished automatically and high quality to help client achieve the goal efficiently for purified antibody products (e.g., IgGs, Fab, scFv, VHH, bi-specific antibodies, etc.) in quantities (i.e., from ug to mg). In order to ensure the high expression level, the automation equipment supporting the batch stability of our high throughput platform.
· Highlights
Fast production starting at 7 BDs
Protein expression & purification experience - 13+ years
Successful projects delivered - 10,000+
Expression success rate - 99%
· One-stop-shop recombinant antibody production process
Antibody type
Expression vectors
CHO, HEK293,Bacteria
Expression
Purification
Report & delivery
· Service SpecificationsService Steps Timeline Description Target types 1-2 weeks • Recombinant proteins
• IgG, Bispecific, Fab, ScFv, VHH, Fc fusion, etc.Gene synthesis & cloning 1-2 weeks Expression plasmids construction Transient expression & purification 1-2 weeks • Purified antibody (SDS-PAGE >95%, endotoxin level <1EU/mg, Including SEC-HPLC report)
• CoA reportNote:
1.Typically, a minimum sample size of 5 is required.
2.Please submit your request to the related BD.
3.The 7 business days is decided from sequence evaluation.
· Case Studies - Abs expression (Data from IgG1 rAb)
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Antibody Production Service- Large Scale Express Platform
Smart Lifesciences not only could provide the HTP&one stop solutions of antibody & protein manufacture services, but also could provide the customized services of protein manufacture & engineering. Especially, we could help to increase customer satisfaction and accomplish the target from the initiate lab R&D stage to industrial scale-up stage by the convenient and efficient methods with a timeline from gene synthesis to antibody delivery of just 10 business days!
The comprehensive antibody development platform provides researchers with stable quality and controllable cost antibody production.
· Highlights
Fast turnaround time, starting at 10 BDs
Protein expression & purification experience - 13+ years
Successful projects delivered - 1,000+
Expression success rate - 99%
· Antibody production process
· Service SpecificationsService Steps Timeline Description Target types Transient expression: 3-4 weeks
Stable expression: 2-3 months• Recombinant proteins
• IgG, Bispecific, Fab, ScFv, VHH, Fc fusion, etc.Gene synthesis & cloning Expression plasmids construction Pilot study (Optional) • Purified antibody (SDS-PAGE >95%, endotoxin level <1EU/mg, including SEC-HPLC report)
• CoA reportPlasmid preparation, transient expression & purification Note:
1. Apply to all types of proteins and antibody fragments.
2. Please submit your request to the related BD.
3. The 10 business days is decided from sequence evaluation.
· Case Studies
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Bacterial Protein Expression
Smart provides recombinant protein expression in E. coli with efficient and cost-effective production. E. coli is a popular host option because of its high yield, low cost, and convenience of manipulation. The E. coli protein expression system is suitable for multiple species, acting as a principal tool for recombinant protein expression and Lifesciencesnological industrial development, especially for small protein production.
E. coli serves as an attractive host system due to its well-characterized genetic background, high yield capacity, robust production cycle, strong anti-pollution abilities, and ease of scaling. Sino Biological offers one-stop services for high-quality E. coli protein expression, with abundant experience in soluble protein expression and production and in protein refolding.
· HighlightsFast turnaround time, starting at 10 BDs
Protein expression & purification experience - 13+ years
Successful projects delivered - 1,000+
Expression success rate - 99%
· Case Studies
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Mammalian Transient Expression
Smart provides mammalian recombinant protein expression services for the different scalable production of proteins. Expecially, the mammalian system is ideal for protein expression of human or other mammalian proteins for proper folding and appropriate post-translational modifications, which support customers developing scientific research, diagnostic assays and research grage proteins.
Our flexibility plaforms helps customers to match the requiring large volume scale-up. We will advice the suitable proposal according to our platform advantages and the request requirements to help customers accomplish the targets, we also will provide the one on one professional and customized service, which will also include the final products, detection reports.
· HighlightsFast turnaround time, starting at 10 BDs
Protein expression & purification experience - 13+ years
Successful projects delivered - 1,000+
Expression success rate - 99%
· Case Studies
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Specialized Antibody Services - Conjugation and Detection
Smart offers specialized antibody services including antibody modification & purification services, and cell line services as well as other antibody services. These specialized antibody services can be further tailored to meet your customize projects and needs.
Antibody Labeling
Antibody biotinylation offers the interchangeability of your choice of labeled avidin, while HRP or fluorescent conjugation provide for simplified direct detection. Either antibody conjugation or labeling service prepares your antibody for both visualization and quantitation of the target molecule.
Antibody Detection
Finally, we offer a wide array of options for antibody detection, such as ELISA, HPLC and FACS. Our antibody detection services offer options to detect any antibody quickly and efficiently.
· Case Studies - Abs conjugation
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AAV Packaging Services
Adeno-associated virus (AAV) is a popular, single-stranded DNA virus that has emerged as an exceptional research tool and leading candidate for genetic payload delivery in gene and cell therapy applications. Several features make AAV the preferred vector for both academic and industry researchers.
· Highlights
Delivery in as little as 12 days from miniprep to purified AAV virus
High purity, low endotoxin levels and minimal empty capsids for reliable performance
Maghabour AAV Purification Kit product for high quality AAV
· Recombinant AAV packaging process
· Service SpecificationsService Steps Timeline Description Gene synthesis & cloning 3-6 weeks •Plasmid contruction AAV packaging & purification •Cotransfection and packing cell line Titering •Concentration and purification
•CoA report (SDS-PAGE, qPCR, titering)Note:
Please submit your request to the related BD.
The 12 business days is decided from sequence evaluation.
· Case Studies - AAV Packaging ServicesCo-transfect wth transfer, packaging and helper plasminds for AAV packaging, collect and purify rAAV virus by Maghabour AAV Purification Kit. QC detection by SDS-PAGE, gPCR and FACS for titer and infectivity determination.
Dilute the AAV unpurified sample and purified samples by a factor of two and five to FACS detection of GFP.
After Maghabour AAV Purification Kit purification the purifed virus retains a high level of infectivity.
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LV Packaging Services
Recombinant lentivirus(LV) is an enveloped retrovirus with a single-stranded RNA genome, which is widely used for efficient gene delivery into mammalian cells. Smart’s current recombinant lentivectors are derived from human immunodeficiency virus-1 (HIV-1) and delivers high quality and high titer LV for overexpression, knockdown and knockout applications.
· Highlights
Fast turnaround
High purity, low endotoxin levels and minimal empty capsids for reliable performance
One stop solution for downstream stable cell line development
· LV packaging process
· Stable Cell Line ServicesStable cell line development could express exogenous genes constitutively, which is an important part for life science research and manufacture. At Smart, we recognize that having strong cell engineering capabilities is critical to support customer’s research. In order to provides the one stop solution from, our experts as well as accompanying technical expertise to help meet customers’ project needs.
· HighlightsOne stop solution
Extensive experience - 13+ years
Project success rate - 98%
· Service SpecificationsService Steps Timeline Description Gene synthesis & cloning 7-11 weeks Plasmid contruction LV packaging & purification Cotransfection and packing cell line Titering detection Concentration and purification
Titering QCStable cell line contruction Virus infection and pressure screening Cell line detection FACS detection of positive results for the cell line and passaging stability Note:
1. Please submit your request to the related BD.
2. The timeline is decided from sequence evaluation.
·Case Studies - LV Packaging ServicesCo-transfect with transfer, packaging and helper plasminds for LV packaging, collect and puriy rAAV virus by PEG method. QC detectionby FACS for titer and infectivity determination.
Constructing the stable cell line by using LV packing method to obtain the stable cell line with only two weeks and the positive rate more than 99%.
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Plasmind Cloning and Preparation
Smart has extensive experience in plasmid DNA production and development, delivering high-quality plasmid preparation services tailored to meet customer needs. Our expertise also extends to patented AAV and LVV helper and packaging plasmids, plasmid cloning and expression, and CRISPR plasmids for precise gene editing.
·Plasmind cloning and preparation process
Fermentation
Purification
Plasminds
