Ni NTA Beads 6FF is based on a highly cross-linked 6% agarose with the same ligand as Ni NTA Beads (See Fig 1.), see table 1. In addition to being able to tolerate harsh reagent conditions (see Table 2), Ni NTA Beads 6FF is more stable due to its pressure-resistant matrix, which can withstand pressures up to 0.3 MPa, making the product is more suitable for industrial large-scale protein purification, which can be achieved at relatively high flow rates for the purification of target proteins.