SpinDesalt Column-3 is a disposable, easy-to-operate purified product suitable for desalting, buffer replacement or small molecule removal of proteins or other large molecule samples with molecular weight greater than 5000Da. The SpinDesalt Column-3 contains 3ml Smartdex G-25. Smartdex G-25 series resin is a kind of dextran as matrix of gel filtration chromatography resin, the main working principle is to use a mesh structure of dextran gel molecular sieves, depending on the molecular size of the separated material for separation. Product use eliminates the need for chromatographs, manual loading, centrifugation operations, waiting for lengthy gravity column passes, sample collection by component, and the ability to process multiple samples in minutes.