rProtein G Beads 4FF is an affinity chromatography medium designed for the purification of monoclonal antibody,polyclonal antibody and Fc tag fusion protein. The recombinant protein G ligand is coupled to highly cross-linked 4% agarose beads. The characteristics of rProtein G Beads 4FF are summarized in Table 1.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also site for albumin and cell-surface binding. The recombinant protein G have been eliminated the nonspecific binding site. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has better affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a.
AbCap G 4FF is a prepacked ready to use ,column for purification of monoclonal and polyclonal antibodies. AbCap G 4FF 1ml and 5ml columns are packed with 1ml and 5ml of rprotein G beads 4FF. AbCap G 4FF can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.