Product Categories
AbCap L 4FF
rProtein L Beads 4FF is an affinity chromatography medium designed for purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein L ligand is immobilized to highly cross-linked 4% agarose beads. The Characteristics of rProtein L Beads 4FF are summarized in Table 1.
Protein L is recombinantly expressed in E. coli and retains the property of binding to the antibody κ-chain without affecting the antigenic binding site of the antibody. Protein L binds well to the kappa light chain of human and mouse, and may bind specifically to certain kappa isoforms of other species. Protein L binds weakly to rabbit immunoglobulins and does not bind bovine, goat or sheep source immunoglobulins.
AbCap L 4FF is a prepacked ready to use ,column for purification of monoclonal and polyclonal antibodies. AbCap L 4FF 1ml and 5ml columns are packed with 1ml and 5ml of rprotein L beads 4FF. AbCap L 4FF can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.
Protein L is recombinantly expressed in E. coli and retains the property of binding to the antibody κ-chain without affecting the antigenic binding site of the antibody. Protein L binds well to the kappa light chain of human and mouse, and may bind specifically to certain kappa isoforms of other species. Protein L binds weakly to rabbit immunoglobulins and does not bind bovine, goat or sheep source immunoglobulins.
AbCap L 4FF is a prepacked ready to use ,column for purification of monoclonal and polyclonal antibodies. AbCap L 4FF 1ml and 5ml columns are packed with 1ml and 5ml of rprotein L beads 4FF. AbCap L 4FF can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.
· Product Description
Table 1. Characteristics of rProtein L Beads 4FF
| Item | Description |
|---|---|
| Matrix Spherical | Highly cross-linked 4% agarose beads |
| Ligand | recombinant protein L |
| Static Binding Capacity | >15mg Mouse IgG/ml medium |
| Particle size | 45-165 μm |
| Maximum Pressure | 0.3 MPa, 3 bar |
| pH | 3-10 |
| Storage Solution | 1×PBS containing 20% ethanol |
| Storage Temperature | 2-8℃ |
| Problem | Possible Cause | Solution |
|---|---|---|
| The flow rate of the column is very low. | The sieve plate is blocked. | Clean or replace the sieve plate. |
| Column is clogged. | Cleaning in place(part 3). | |
| Filtering the sample solution by passing them through a 0.22 μm or 0.45 μm filter. | ||
| The curve is not stable during sample purification | Tiny air bubbles from buffer or sample. | De-gas buffers and samples. Do not allow the column to dry. |
| A considerable amount of sample has been loaded, but no specific antibody of interest is detected. | The concentration of antibody of interest is very low. | Purify the antibody using the specific antigen coupled to a beads (i.e., PabPur Sufolink Beads, Cat. No. SA018005). |
| The antibody is sensitive to low-pH elution buffer | Neutralize the eluted fractions with Neutralization Buffer immediately after elution. | |
| The recovery rate gradually decreases. | The sample is overloaded. | Reduce the loading volume. |
| The column is too dirty and the binding capacity is reduced. | Cleaning in place (part 3). |
· Ordering Information
| Product | Cat. No. | Size |
|---|---|---|
| rProtein L Beads | SA045005 | 5 ml |
| SA045025 | 25 ml | |
| SA045100 | 100 ml | |
| SA045500 | 500 ml | |
| SA04501L | 1 L | |
| SA04510L | 10 L | |
| rProtein L Beads 4FF | SA033005 | 5 ml |
| SA033025 | 25 ml | |
| SA033100 | 100 ml | |
| SA033500 | 500 ml | |
| SA03301L | 1 L | |
| SA03310L | 10 L | |
| AbCap L 4FF | SA033C11 | 1×1 ml |
| SA033C51 | 5×1 ml | |
| SA033C15 | 1×5 ml | |
| SA033C55 | 5×5 ml | |
| SA033CS | 3×1 ml+1×5 ml |
· Download Manual
· Related Products
