The human c-Myc gene is a key member of the Myc gene family and is associated with the development of various tumors. Myc is a relatively large protein, with human Myc protein having a molecular weight ranging from 50-70 kDa. The Myc tag is composed of ten amino acids (EQKLISEEDL) derived from amino acids 410-419 of the human Myc protein, and this peptide has a molecular weight of approximately 1.2 kDa. Using recombinant DNA technology, it can be fused to the N- or C-terminus of a target protein, facilitating its purification and detection. Anti-Myc antibodies can be used to detect the expression and intracellular localization of Myc-tagged fusion proteins, as well as for their purification, qualitative, or quantitative analysis. Horseradish Peroxidase (HRP) is conjugated to the anti-Myc antibody through a specific process. In Western blot (WB) experiments, upon adding chemiluminescent substrates A and B (typically luminol and hydrogen peroxide), the luminol reacts with hydrogen peroxide under the catalytic action of HRP to form an unstable peroxide intermediate. This intermediate decomposes, generating an electronically excited species. When this species returns to the ground state, light is emitted, allowing for rapid detection of the Myc-tagged fusion protein.