Product Categories
Co NTA Beads 6FF
Co NTA Beads 6FF is intended for preparative purification of histidine-tagged recombinant proteins from all prokaryotic and eukarotic expression systems. Co NTA Beads 6FF consists of highly cross-linked 6% agarose with an immobilized chelating group. The talon ligand is a tetra-dentate chelator charged with cobalt. Co NTA Beads 6FF offers enhanced selectivity for histidine-tagged proteins compared to nickel-charged medium.
· Product Description
| Item | Description |
|---|---|
| Matrix Spherical | Highly cross-linked 6% agarose |
| Precharged ion | Cobalt ion |
| Static Binding Capacity | >20mg 6×His-tagged protein/ml medium |
| Particle size | 45-16μm |
| Maximum Pressure | 0.3MPa, 3bar |
| Storage Solution | 1×PBS containing 20% ethanol |
| Storage Temperature | 4-30°C |
| Reagent | Stability |
|---|---|
| Reductants | 10 mM β-mercaptoethanol1 |
| Denaturants | 8 M urea |
| 6 M Gua-HCl | |
| Detergent | < 1% Triton™ X-100 |
| 1% NP-40 | |
| 1% CHAPS, SDS, sarcosyl | |
| Other additives | ≤500 mM imidazole2 at pH7.0 to 8.0 for elution |
| 30% ethanol3 | |
| 20% glycerol | |
| 500 mM KCl | |
| 1.0 M NaCl | |
| 20mM MES | |
| 50 mM Tris4 | |
| 50 mM HEPES | |
| 50 mM MOPS |
· Ordering Information
| Product | Cat. No. | Size |
|---|---|---|
| Co NTA Beads | SA037005 | 5 ml |
| SA037025 | 25 ml | |
| SA037100 | 100 ml | |
| SA037500 | 500 ml | |
| SA03701L | 1 L | |
| SA03710L | 10 L | |
| Co NTA Beads 6FF | SA038005 | 5 ml |
| SA038025 | 25 ml | |
| SA038100 | 100 ml | |
| SA038500 | 500 ml | |
| SA03801L | 1 L | |
| SA03810L | 10 L | |
| HisCap Co 6FF | SA038C11 | 1×1 ml |
| SA038C51 | 5×1 ml | |
| SA038C15 | 1×5 ml | |
| SA038C55 | 5×5 ml | |
| SA038CS | 3×1 ml+1×5 ml | |
| Co Smart Beads 6FF | SA062005 | 5 ml |
| SA062025 | 25 ml | |
| SA062100 | 100 ml | |
| SA062500 | 500 ml | |
| SA06201L | 1 L | |
| SA06210L | 10 L |
· Download Manual
· Related Products
