Molecular-Diagnostic Magnetic Beads include Magsilica Beads, Streptavidin Magpoly Beads, DNA FragSelect XP Magnetic Beads, and Oligo(dT)25 MagPoly Beads, they are widely used for nucleic acid purification and fragment sorting.

Oligo(dT)25 MagPoly Beads (Cat. No.: MP0301) :Prepared by reacting biotinylated Oligo(dT)25 with Streptavidin Magpoly Beads(Cat. No.: MP0404).

Workflow: Total RNA( isolated from mouse liver) is first hybridized with a biotin-labeled oligodeoxynucleotide. The Oligo(dT)25 MagPoly Beads then specifically capture mRNA or remove rRNA, achieving mRNA purification.

1. High binding capacity for biotin-modified ligands

As shown in the Figure 1, the Ct values of mRNA before and after purification are essentially consistent, indicating that the purification efficiency of mRNA is close to 100% and the non-specific binding is very low. (Actual results may vary due to differences in experimental conditions, detection instruments, etc. The data in the figure are for reference only.)

Figure 1. Results of using Oligo (dT)25 Magnetic Beads for purifying mRNA from total RNA( mouse liver).

2. Good batch-to-batch stability

Three batches of Oligo(dT)25 MagPoly Beads were each incubated and hybridized with the same total RNA sample to test mRNA and rRNA capture. CT value comparison with the sample indicates low batch-to-batch variation in mRNA purification, demonstrating high application stability.