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HisCap Smart 6FF
Ni Smart Beads 6FF is a new immobilized metal ion affinity chromatography(IMAC)medium precharged with nickel ions. Ni Smart Beads 6FF is designed mainly for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. The strong nickel ion binding also provides very high resistance to EDTA and reducing agents like DTT. Ni Smart Beads 6FF enable direct loading of large sample volumes without having to remove agents that cause stripping of nickel ions from conventional IMAC medium. Ni Smart Beads 6FF is stable in all buffers commonly used in IMAC. HisCap Smart 6FF is one of a range of prepacked, ready-to-use columns for affinity chromatography. It is prepacked with 1ml and 5ml of Ni Smart Beads 6FF. Five different packing sizes are available. HisCap Smart 6FF has the standard interface and can be operated with a peristaltic pump or liquid chromatography systems, such as ÄKTA. It is fast, simple and easy operation.
· Product Description
Table 1. Characteristics of Ni Smart Beads 6FF
| Item | Description |
|---|---|
| Matrix Spherical | Highly cross-linked 6% agarose |
| Static Binding Capacity | >10mg 6×His-tagged protein/ml medium |
| Particle size | 45-165μm |
| Column Size | 1ml 0.7X2.5cm |
| 5ml 1.6X2.5cm | |
| Maximum Pressure | 0.3MPa, 3bar |
| Storage Solution | 1×PBS containing 20% ethanol |
| Storage Temperature | 4-30°C |
Table 2. Chemical compatibilities for Ni Smart Beads 6FF
| Solution | Time |
|---|---|
| 0.01M HCl ,0.01M NaOH | One week |
| 10mM EDTA, 1M NaOH, 5mM DTT, 5mM TCEP, 20mM β-mercaptoethanol, 6M guanidine-HCl | 24 hours |
| 500mM imidazole, 100mM EDTA | 2 hours |
| 30% isopropanol | 20 minutes |
· Ordering Information
| Product | Cat. No. | Size |
|---|---|---|
| Ni Smart Beads | SA035005 | 5 ml |
| SA035025 | 25 ml | |
| SA035100 | 100 ml | |
| SA035500 | 500 ml | |
| SA03501L | 1 L | |
| SA03510L | 10 L | |
| Ni Smart Beads 6FF | SA036005 | 5 ml |
| SA036025 | 25 ml | |
| SA036100 | 100 ml | |
| SA036500 | 500 ml | |
| SA03601L | 1 L | |
| SA03610L | 10 L | |
| HisCap Smart 6FF | SA036C11 | 1×1 ml |
| SA036C51 | 5×1 ml | |
| SA036C15 | 1×5 ml | |
| SA036C55 | 5×5 ml | |
| SA036CS | 3×1 ml+1×5 ml |
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