AbCap AG 4FF
rProtein A/G Beads 4FF is an affinity chromatography medium designed for the purification of monoclonal antibody and polyclonal antibody at both laboratory and process acale. The rProtein A/G is coupled to highly cross-linked 4% agarose beads by a technique which generates a stable thioether linkage between rProtein A/G and the agarose beads. The coupling technique is optimized to give a high binding capacity for IgG.This binding capacity,together with the excellent kinetic and flow properties of the highly cross-linked beads.rProtein A/G Beads 4FF can be used for antibody purification, immuneprecipitation and Co- immuneprecipitation.
· Product Description

Table 1. Characteristics of rProtein A/G Beads 4FF

ItemDescription
Matrix SphericalHighly cross-linked 4% agarose beads
Ligandrecombinant protein A/G
Static Binding Capacity10-15 mg Rabbit IgG/ml medium
Particle size45-165 μm
Maximum Pressure0.3 MPa, 3 bar
pH3-10
Storage Solution1×PBS containing 20% ethanol
Storage Temperature2-8℃

Table 2. Relative binding strengths of antibodies from various species to protein G protein A and protein A/G as measured in a competitive ELISA test.

SpeciesSubclassProtein AProtein GProtein A/G
HumanIgAvarible—++
IgD———
IgE———
IgG1++++++++++++
IgG2++++++++++++
IgG3—++++++++
IgG4++++++++++++
IgMvariable—++
Avian egg yolkIgY———
Cow
++++++++++
Dog
++++++++++
Goat
—++++++++
Guinea pigIgG1++++++++++
IgG2++++++++++
Hamster
+++
HorseTotal IgG++++++++++
Koala
—+
Llama
—+
Monkey(rhesus)
++++++++++++
MouseIgG1+++++++
IgG2a++++++++++++
IgG2b+++++++++
IgG3++++++++
IgMvariable——
Pig
++++++++++
RabbitTotal IgG+++++++++++
RatIgG1—+++
IgG2a—++++++++
IgG2b—++++
IgG3+++++
SheepTotal IgG+/-++++

++++=strong binding; ++= medium binding; —=weak binding or no binding

· Ordering Information
ProductCat. No.Size
rProtein A/G Beads 4FFSA0320055 ml
SA03202525 ml
SA032100100 ml
SA032500500 ml
SA03201L1 L
SA03210L10 L
AbCap A/G 4FFSA032C111 X 1 ml
SA032C515 X 1 ml
SA032C151 X 5 ml
SA032C555 X 5 ml
SA032CS3X1 ml+1X5 ml
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