This product is a chemiluminescence reagent kit based on a luminol substrate, which can be catalyzed by horseradish peroxidase (HRP) to produce light emission. The kit features an optimized substrate composition and utilizes a novel high-efficiency enhancer, resulting in a luminescence intensity 30-100 times higher than that of traditional ECL developing solutions while effectively reducing background interference. By employing a new oxidizer in place of unstable hydrogen peroxide, the kit exhibits enhanced stability and can be stored at room temperature for up to one year. When the working detection solution is catalyzed by HRP, it emits fluorescence at specific wavelengths (400-450 nm), suitable for exposure on X-ray films or direct scanning with a fluorescence CCD. It is primarily designed for applications in Western blot detection and chemiluminescent immunoassay systems.