β-Tubulin, also known as tubulin, is a cytoskeletal protein that exists in two forms: α-tubulin and β-tubulin. These two subunits bind tightly to form a heterodimer. Comprising 455 amino acids with a molecular weight of 55 kDa, β-tubulin is characterized by stable expression levels. Due to this consistency, it is widely utilized as a loading control in Western blotting (WB) and is frequently employed in immunostaining to visualize microtubule structures within cells.