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HisCap Co 6FF
Co NTA Beads 6FF is intended for preparative purification of histidine-tagged recombinant proteins from all prokaryotic and eukarotic expression systems. Co NTA Beads 6FF consists of highly cross-linked 6% agarose with an immobilized chelating group. The talon ligand is a tetra-dentate chelator charged with cobalt. Co NTA Beads 6FF offers enhanced selectivity for histidine-tagged proteins compared to nickel-charged medium. The characteristics of Co NTA Beads 6FF are summarized in Table 1. HisCap Co 6FF is one of a range of prepacked, ready-to-use columns for affinity chromatography. It is prepacked with 1ml and 5ml of Co NTA Beads 6FF. Five different packing sizes are available. HisCap Co 6FF has the standard interface and can be operated with a peristaltic pump or liquid chromatography systems, such as ÄKTA. It is fast, simple and easy operation.
· Product Description
Table 1. Characteristics of Co NTA Beads 6FF
| Item | Description |
|---|---|
| Matrix Spherical | Highly cross-linked 6% agarose |
| Precharged ion | Cobalt ion |
| Static Binding Capacity | >20mg 6×His-tagged protein/ml medium |
| Particle size | 45-16μm |
| Maximum Pressure | 0.3MPa, 3bar |
| Storage Solution | 1×PBS containing 20% ethanol |
| Storage Temperature | 4-30°C |
Table 2. Chemical compatibilities for Co NTA Beads 6FF
| Reagent | Stability |
|---|---|
| Reductants | 10 mM β-mercaptoethanol1 |
| Denaturants | 8 M urea |
| 6 M Gua-HCl | |
| Detergent | < 1% Triton™ X-100 |
| 1% NP-40 | |
| 1% CHAPS, SDS, sarcosyl | |
| Other additives | ≤500 mM imidazole2 at pH7.0 to 8.0 for elution |
| 30% ethanol3 | |
| 20% glycerol | |
| 500 mM KCl | |
| 1.0 M NaCl | |
| 20mM MES | |
| 50 mM Tris4 | |
| 50 mM HEPES | |
| 50 mM MOPS |
· Ordering Information
| Product | Cat. No. | Size |
|---|---|---|
| Co NTA Beads | SA037005 | 5 ml |
| SA037025 | 25 ml | |
| SA037100 | 100 ml | |
| SA037500 | 500 ml | |
| SA03701L | 1 L | |
| SA03710L | 10 L | |
| Co NTA Beads 6FF | SA038005 | 5 ml |
| SA038025 | 25 ml | |
| SA038100 | 100 ml | |
| SA038500 | 500 ml | |
| SA03801L | 1 L | |
| SA03810L | 10 L | |
| HisCap Co 6FF | SA038C11 | 1×1 ml |
| SA038C51 | 5×1 ml | |
| SA038C15 | 1×5 ml | |
| SA038C55 | 5×5 ml | |
| SA038CS | 3×1 ml+1×5 ml | |
| Co Smart Beads 6FF | SA062005 | 5 ml |
| SA062025 | 25 ml | |
| SA062100 | 100 ml | |
| SA062500 | 500 ml | |
| SA06201L | 1 L | |
| SA06210L | 10 L |
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