Adeno-associated virus (AAV) is a popular, single-stranded DNA virus that has emerged as an exceptional research tool and leading candidate for genetic payload delivery in gene and cell therapy applications. Several features make AAV the preferred vector for both academic and industry researchers.
Delivery in as little as 12 days from miniprep to purified AAV virus
High purity, low endotoxin levels and minimal empty capsids for reliable performance
Maghabour AAV Purification Kit product for high quality AAV
| Service Steps | Timeline | Description |
|---|---|---|
| Gene synthesis & cloning | 3-6 weeks | •Plasmid contruction |
| AAV packaging & purification | •Cotransfection and packing cell line | |
| Titering | •Concentration and purification •CoA report (SDS-PAGE, qPCR, titering) |
Note:
Please submit your request to the related BD.
The 12 business days is decided from sequence evaluation.
Co-transfect wth transfer, packaging and helper plasminds for AAV packaging, collect and purify rAAV virus by Maghabour AAV Purification Kit. QC detection by SDS-PAGE, gPCR and FACS for titer and infectivity determination.
Dilute the AAV unpurified sample and purified samples by a factor of two and five to FACS detection of GFP.
After Maghabour AAV Purification Kit purification the purifed virus retains a high level of infectivity.