CRO Services
Polyclonal Antibody Discovery
Smart Lifesciences comprehensive polyclonal antibody services include production of custom polyclonal antibodies in mouse, rabbit and other species. Smart provides one-stop solutions for your custom polyclonal antibody production, taking your project all the way from antigen design, peptide synthesis, and animal immunization, to antibody purification and QC with ELISA test.
· Highlights
One stop solution
R&D carrier protein
High purification yield
High success rate
· Polyclonal antibody discovery process
Antigen preparation
Immunization
B cell isolation
Screening
· Service Specifications
| Phases | TAT(weeks) | Descriptions |
|---|---|---|
| Phase 0: Antigen preparation | ||
| Peptide/Protein preparation | 3-4 | / |
| Phase I: Animal immunization | ||
| Three immunizations with conventional method (two rabbits) & report only | 6~8 | ELISA report after the 3rd immunization |
| Phase II: Polyclonal antibody purification | ||
| Purification for all rabbits by antigen affinity column | 2 | Purified antibody from both rabbits and ELISA report |
Note:
1.Starting material: antigen protein>5mg, purity>85%, conc.>1mg/ml.
2.Starting material: antigen peptide>15mg, purity>90%.
· Project case study - Target A rabbit pAb
