At Smart Lifesciences, has developed proprietary procedures for nanobody discovery. We optimized the immunization schedule and screening proceddes to target-focused immune response and ensure the successful isolation of high-affinity alpaca nanobody within a short turnaround time.
Advantages of nanobody include, stability at hight temperature & pH, VHHs could recognize antigenic sites that are normally not recognized by IgG antibodies, the small moleculara size could help with rapid tissue penetration and conjugation applications, low cost for large-scale production. All positice VHH generated under your project will belong to you.
High throughput and shot timeline - single cell within 1 week
Animal immunization (carrier protein to increase the anti-serum titer more than 20%, self-developed adjuvant)
Project success rate - 100%.
One stop solution - from gene to antibody
Extensive experience - 13+ years
Antigen preparation
Immunization
B cell isolation
Screening
lgG sequencing
rAb expression
| Phases | TAT(weeks) | Descriptions |
|---|---|---|
| Phase 0: Antigen preparation | ||
| Peptide/Protein preparation | 3-4 | / |
| Phase I: Animal immunization | ||
| Three immunizations with conventional method & report only | 8-10 | ELISA report after the 3rd immunization |
| Serum collection and PBMC isolation | 8-10 | ELISA report after the 3rd immunization |
| Phase II: Positive clones screening | ||
| Option 1: Phage display and bio-panning | 2-8 | ELISA report of positive clones |
| Option 2: B cell cloning & ELISA serttning | 2-8 | ELISA report of positive clones |
| Phase III:Sequencing, recombinant VHH expression and validation | ||
| Antibody sequencing and construction | 2-4 | Antibody sequencing and alignment reports |
| Small scale recombinant VHH production and validation | 2-4 | 0.1mg recombinant VHH/clone (SDS-PAGE>90%, endotoxin level<5EU/mg) |
Note:
1.Starting material: antigen protein>5mg, purity>85%, conc.>1mg/ml.
2.Starting material: antigen peptide>15mg, purity>90%.
