Smart's customized rabbit monoclonal antibody development service could offer high affinity, quality, specific, and sensitive monoclonal antibodies against a wide range of antigens. By using proprietary B cell cloning technology, this service provides several advantages over traditional mouse monoclonal antibody development.
Smart’s B-cell cloning technology has a high success rate against difficult targets such as PTM site, small molecule,antibody drugs, etc. Our advanced, proprietary rabbit monoclonal antibody discovery technology involves the isolation and cell culture of rabbit B cells to generate high-specificity, high-affinity antibodies to meet customers’ downstream applications.
In a word, this platform could offer higher specificity, affinity and high success rate, the ability to recognize more epitopes, and the capability to large scale antibody production and wide applications in scientific research, diagnostics, and therapeutics.
Animal immunization (carrier protein to increase the anti-serum titer more than 20%, self-developed adjuvant)
Low price - almost same with mouse mAb
Project success rate - 100%
One stop solution - from gene to antibody
Extensive experience - 13+ years
Antigen preparation
Immunization
B cell isolation
Screening
lgG sequencing
rAb expression
| Phases | TAT(weeks) | Descriptions |
|---|---|---|
| Phase 0: Antigen preparation | ||
| Peptide/Protein preparation | 3-4 | / |
| Phase I: Animal immunization | ||
| Three immunizations with conventional method (three rabbits) & report only | 6~8 | ELISA report after the 3rd immunization |
| Phase II: B cell cloning and screening | ||
| One round of B cell cloning&report only | 2 | Positive clone report for up to 20 clones |
| Phase III:Sequencing, validation and recombinant antibody production | ||
| Antibody sequencing and validation | 2 | Antibody sequencing and alignment reports for up to 5 clones |
| Small scale recombinant antibody production for five clones | 3~4 | 0.1mg recombinant antibody/clone from up to 5 clones |
| Optional: Antibody sequencing and small scale antibody production for one extra clone | / | Antibody sequencing and alignment report and 0.1mg recombinant antibody/clone |
| Phase IV: Recombinant Antibody production | ||
| Option1: Recombinant Antibody production | 3 | 0.1-0.5mg/clone |
| Option2: Recombinant Antibody production | 4~6 | 1-2mg/clone |
Note:
1.Starting material: antigen protein>5mg, purity>85%, conc.>1mg/ml.
2.Starting material: antigen peptide>15mg, purity>90%.
