The mouse monoclonal antibody is a type of antibody produced by a single clone after immunization derived from mice. Monoclonal antibodies have a much higher specificity and lower between-batch variability compared to pAbs, which is widely used in such as research and development (R&D), diagnostics, and therapeutics.
Smart's custom mouse monoclonal antibody development service provides a comprehensive solution for generating high-quality monoclonal antibodies against any target of interest. The service includes immunogen design and synthesis, immunization, hybridoma generation, screening, and antibody production.With Smart's fully customizable monoclonal antibody generation services, you have complete control over every stage of the process, allowing you to modify your approach, restart a phase, or terminate the project if necessary. Avoid the risks associated with a rigid protocol and choose our fully customizable service that provides the ultimate flexibility. Partnering with Smart's years of expertise in custom antibody development can help you achieveproject success.
Animal immunization (carrier protein to increase the anti-serum titer more than 20%, self-developed adjuvant)
Project success rate - 100%
One stop solution - from gene to antibody
Extensive experience - 13+ years
Antigen preparation
Immunization
Screening
B cell isolation
lgG sequencing
rAb expression
| Phases | TAT (weeks) | Descriptions |
|---|---|---|
| Phase 0: Antigen preparation | ||
| Peptide/Protein preparation | 3-4 | / |
| Phase I: Animal immunization | ||
| Three immunizations with conventional method (five mice) | 6-8 | ELISA report after the 3rd immunization |
| Phase II: Cell fusion and sub cloning screening | ||
| One cell fusion with up to 15 plates | 5 | Positive clone report for up to 10 clones 2 vials of each sub-cloned hybridoma cell line from 3-5 positive primary cell lines |
| Subcloning for 3-5 clones | ||
| Phase III: Antibody production | ||
| Option 1: Antibody production in low IgG culture medium and protein A/G purification | 3 | 0.1-0.5mg/clone |
| Option 2: Ascites production and protein A/G purification | 2-3 | 1-2mg/clone |
Note:
1.Starting material: antigen protein>3mg, purity>85%, conc.>1mg/ml.
2.Starting material: antigen peptide>10mg, purity>90%.
